Sarcelle
09.08.2007, 20:40
Molecular analysis of highly pathogenic avian influenza virus of subtype H5N1 isolated from wild birds and mammals in northern Germany
http://vir.sgmjournals.org/cgi/content/abstract/88/2/554
Siegfried Weber1, Timm Harder2, Elke Starick2, Martin Beer2, Ortrud Werner2, Bernd Hoffmann2, Thomas C. Mettenleiter1 and Egbert Mundt1,
1 Institute of Molecular Biology, Friedrich-Loeffler-Institut, 17493 Greifswald–Insel Riems, Germany
2 Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, 17493 Greifswald–Insel Riems, Germany
Correspondence
Egbert Mundt
emundt@uga.edu
Analysis of the full-length sequences of all eight segments of the German wild-bird H5N1 highly pathogenic avian influenza virus index isolate, A/Cygnus cygnus/Germany/R65/2006, and an H5N1 isolate from a cat (A/cat/Germany/R606/2006) obtained during an outbreak in February 2006 revealed a very high similarity between these two sequences. One amino acid substitution in the PA gene, encoding a protein involved in virus RNA replication, and one amino acid substitution in the haemagglutinin (HA) protein were observed. Phylogenetic analyses of the HA and neuraminidase nucleotide sequences showed that avian influenza H5N1 isolates from the Astrakhan region located in southern Russia were the closest relatives. Reassortment events could be excluded in comparison with other ‘Qinghai-like’ H5N1 viruses. In addition, an H5N1 isolate originating from a single outbreak in poultry in Germany was found to be related closely to the H5N1 viruses circulating at that time in the wild-bird population.
http://vir.sgmjournals.org/cgi/content/abstract/88/2/554
Siegfried Weber1, Timm Harder2, Elke Starick2, Martin Beer2, Ortrud Werner2, Bernd Hoffmann2, Thomas C. Mettenleiter1 and Egbert Mundt1,
1 Institute of Molecular Biology, Friedrich-Loeffler-Institut, 17493 Greifswald–Insel Riems, Germany
2 Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, 17493 Greifswald–Insel Riems, Germany
Correspondence
Egbert Mundt
emundt@uga.edu
Analysis of the full-length sequences of all eight segments of the German wild-bird H5N1 highly pathogenic avian influenza virus index isolate, A/Cygnus cygnus/Germany/R65/2006, and an H5N1 isolate from a cat (A/cat/Germany/R606/2006) obtained during an outbreak in February 2006 revealed a very high similarity between these two sequences. One amino acid substitution in the PA gene, encoding a protein involved in virus RNA replication, and one amino acid substitution in the haemagglutinin (HA) protein were observed. Phylogenetic analyses of the HA and neuraminidase nucleotide sequences showed that avian influenza H5N1 isolates from the Astrakhan region located in southern Russia were the closest relatives. Reassortment events could be excluded in comparison with other ‘Qinghai-like’ H5N1 viruses. In addition, an H5N1 isolate originating from a single outbreak in poultry in Germany was found to be related closely to the H5N1 viruses circulating at that time in the wild-bird population.